Intermediate

Part:BBa_K568005:Design

Designed by: Team TU Munich 2011   Group: iGEM11_TU_Munich   (2011-09-14)
Revision as of 22:10, 20 September 2011 by Sue (Talk | contribs) (→‎Design Notes)

Intermediate optogenetical AND-Gate without T7ptag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 644
    Illegal XhoI site found at 1786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 971
    Illegal AgeI site found at 3817
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3820


Design Notes

Red light sensor (BBa_K568000) added to intermediated part of optogenetic AND gate (BBa_K568006). Next step is cloning of T7ptag (BBa_K228000 at 3' site of intermediate construct.

Source

tbd

References