Intermediate

Part:BBa_K568005

Designed by: Team TU Munich 2011   Group: iGEM11_TU_Munich   (2011-09-14)
Revision as of 22:04, 20 September 2011 by Sue (Talk | contribs)

Intermediate optogenetical AND-Gate without T7ptag

Cloning intermediate. Last step of assembly. This is an intermediate cloning construct. For the final part (BBa_K568001) T7ptag, a T7 polymerase with amber stop codon mutation, (BBa_K228000) needs to be added.

Usage and Biology

see Part (BBa_K568001)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 644
    Illegal XhoI site found at 1786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 971
    Illegal AgeI site found at 3817
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3820


[edit]
Categories
Parameters
n/aIntermediate optogenetical AND-Gate without T7ptag