Signalling

Part:BBa_K608101:Design

Designed by: Julia Mller   Group: iGEM11_Freiburg   (2011-09-14)
Revision as of 10:15, 14 September 2011 by Juliimapril (Talk | contribs) (→‎References)

CcaR, green light response regulator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 380
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We deleted an EcoRI restriction site in CcaR, in order to make it compatible to the iGEM standart.


Source

It was PCR-amplified from the genomic sequence of Synechocystis sp. PCC 6803. The following primers were used: XbaI_RBS_ccaR_up, TATTCTAGATTCACACAGGAAACCTCACTAATGAGAATTCTTTTAGTGGAGGATG and ccaR_SpeI_PstI_dw,GCCGGACTGCAGCGGCCGCTACTAGTCTAGTTTTTCCCTTGGCACAAAG

References

Yuu Hirose et al ( 2008 )"Cyanobacteriochrome CcaS is the green light receptor that induces the expression of phycobilisome linker protein."
Proc Natl Acad Sci U S A. 105: 9528–9533.