Part:BBa_K515107

Composite p(tetR) - Dendra2

Description

Biobrick <a href="https://parts.igem.org/Part:BBa_K515007">BBa_K515007</a>, a translational subunit under the control of promoter Dendra is a photoconvertible protein. In its natural state, it is excited and emits at 486 and 505nm, respectively. It can be converted to be excited at 558nm and consequently emit at 575nm by single-photon stimulation. Conversion can be efficiently achieved at wavelengths of both 488 and 405nm (Gurskaya et al., 2006). However, the cytotoxic wavelength of 405 converts Dendra more efficiently (data not shown).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Characterisation

Dendra can be efficiently converted using confocal microscopy.