Part:BBa_K607003

Prm_GFP-LVA

GFP with LVA-tag under transcriptional control of a modified Prm(PcI) promoter. The Prm promotor is induced by cI. For more information on cI, see: https://parts.igem.org/wiki/index.php?title=Part:BBa_K607001
The Prm promotor is part of an operator system where cI or cro can be produced.

cI dimers will bind to the first two operators. This will look like this:

The “free” PRM promoter will subsequently be occupied by RNA polymerase which in turn will transcribe cI. This leads to a continuous transcription (DNA to RNA) and subsequent expression (RNA to protein) of cI. This means cI is regulating its own synthesis and simulataneously maintaining the repression of cro. Conversely this auto-regulation can also be obeserved if cro is abundant: cro dimer binding to the operator sites will block binding of RNA polymerase to PRM, but will allow binding to PR, which in turn will lead exclusively to cro transcription. At increasing concentrations of cI the probability of cI binding to OR3 also increases, inhibiting therefore RNA polymerase binding to PRM. This means at higher concentrations cI is inhibiting its own synthesis, acting as a self-repressor.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 752