Reporter
Luc

Part:BBa_K525999:Design

Designed by: Anna Drong   Group: iGEM11_Bielefeld-Germany   (2011-09-13)
Revision as of 17:23, 16 September 2011 by Jaretz (Talk | contribs)

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Firefly -Luciferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 1657
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 814


Design Notes

Firefly luciferase in Freiburg BioBrick assembly standard (RFC 25) as an enzymatic reporter domain


Source

  • organism: Photinus pyralis
  • Created via PCR on BBa_I712019 which was made from Promega's pGL3 plasmid


References