Reporter
Luc
Part:BBa_K525999:Design
Designed by: Anna Drong Group: iGEM11_Bielefeld-Germany (2011-09-13)
Firefly -Luciferase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 1657 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 814
Design Notes
Firefly luciferase in Freiburg BioBrick assembly standard (RFC 25) as an enzymatic reporter domain
Source
- organism: Photinus pyralis
- Created via PCR on BBa_I712019 which was made from Promega's pGL3 plasmid