Composite

Part:BBa_K515100:Design

Designed by: Atipat Patharagulpong   Group: iGEM11_Imperial_College_London   (2011-09-06)
Revision as of 10:12, 13 September 2011 by Nikkikapp (Talk | contribs) (→‎Design Notes)

IAA biosynthetic genes under control of the Pveg2 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 547
    Illegal BamHI site found at 1492
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 254
    Illegal NgoMIV site found at 2835
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

An insulator sequence has been designed before each RBS to contain no homology with the vector, thereby avoiding recombination and allowing easier PCR removal of the individual parts from the device as well as promoter switching without influencing the RBS.

The coding regions have been optimised for B. subtilis and E. coli through the use of the program we have designed.

The parts were assembled into pSB1C3 by CPEC assembly.

Source

Genomics sequence originating from P. savastanoi, synthesized by Eurofins.

References