Part:BBa_K549005
LacI promotor fused with the iron dependent regulator fur
The biobrick contains the lacI promotor from E. coli fused with the iron dependent regulator fur from Neisseria meningitidis. Fur regulates genes via binding at fur-binding-boxes. The backbone pSB3C5 is compartible to the standart backbone pSB1C3.
The sequence of fur is missing in the sequence part of this page. fur follows downstream of the RBS with the following sequence: ATGGAAAAATTCAACAATATTGCACAACTGAAAGACAGCGGTCTGAAGGTTACCGGCCCG CGTTTGAAGATTTTGGATTTGTTCGAGACGCATGCGGAAGAGCATTTGAGTGCGGAAGAT GTGTACCGCATTTTGTTGGAAGAGGGTGTGGAAATCGGTGTGGCGACGATTTACCGTGTG CTGACCCAGTTTGAGCAGGCGGGCATTTTGCAACGCCATCATTTTGAAACGGGCAAGGCG GTTTATGAGTTGGACAAAGGCGACCACCATGACCACATCGTCTGCGTGAAGTGCGGCGAG GTAACGGAGTTCCACAATCCCGAAATCGAAGCCCTGCAAGACAAAATCGCGGAAGAAAAC GGCTACCGCATCGTCGATCACGCGCTTTATATGTACGGCGTGTGCAGCGACTGTCAGGCC AAGGGCAAACGTTAA
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 323
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