Part:BBa_K515000

IaaM - tryptophan-2-mono-oxygenase

IAA tryptophan monooxygenase (IaaM) catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which acts as an intermediate to the production of indole-3-acetic acid in the IAM pathway. There are several different pathways to produce indole-3-acetic acid. [1] We are using genes from the IAM pathway (IaaM and IaaH) of the plant pathogen, Psuedomonas savastanoi to produce indole-3-acetic acid in E. coli </p>

tryptophan --> indole acetamide --> indole-3-acetic acid

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 450
  Illegal BamHI site found at 1395
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 157
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1]Spaepen S. et al., 2007. Indole-3-acetic acid in microbial and microorganism-plant signaling. Federation of European Microbiological Societies Microbiology Reviews , 31, pp.425–448