Part:BBa_K538311

Cpn60/10 operon (pBAD)

Cpn60/10 K538311

This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: icE. coli. Its purpose is to express the Cpn60/10 system from Polaribacter irgensii, by simultaneously expressing Cpn10 and Cpn60 (BBa_K538000 and BBa_K538001, respectively). When expressed individually, these chaperones haven't been observed to significantly affect E. coli, but when expressed together, they strongly increase E. coli's growth rate at suboptimal temperatures. Team Amsterdam submitted 4 operon-like Bricks that facilitate Cpn60/10 coexpression. These are:

• BBa_K538210: pLacI + RBS + Cpn10 + RBS + Cpn60
• BBa_K538211: pLacI + RBS + Cpn60 + RBS + Cpn10
• BBa_K538310: pBAD + RBS + Cpn10 + RBS + Cpn60
• BBa_K538311: pBAD + RBS + Cpn60 + RBS + Cpn10

The first two are controlled by the Lac operon's promoter, pLacI (BBa_R0010). The latter two are placed under control of a strongly arabinose-induced promoter, derived from pBAD by team [http://2010.igem.org/Team:British_Columba British Columbia 2009]. The parts under control of the same promoters aren't expected to be functionally different, but they were designed like this regardless, so one could be used even if assembly of the other failed.

Refer to the main pages of this part's coding regions (Cpn10: BBa_K538000 and Cpn60: BBa_K538001) for more information on how their expression facilitates cold resistance in E. coli.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 125
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1026
  Illegal BamHI site found at 65
  Illegal BamHI site found at 1092
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1884