Part:BBa_K538210

Cpn10/60 operon (pLacI)

Cpn10/60 K538210

This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: icE. coli. Its purpose is to express the Cpn60/10 system from Polaribacter irgensii, by simultaneously expressing Cpn10 and Cpn60 (BBa_K538000 and BBa_K538001, respectively). When expressed individually, these chaperones haven't been observed to significantly affect E. coli, but when expressed together, they strongly increase E. coli's growth rate at suboptimal temperatures. Team Amsterdam submitted 4 operon-like Bricks that facilitate Cpn60/10 coexpression. These are:

• BBa_K538210: pLacI + RBS + Cpn10 + RBS + Cpn60
• BBa_K538211: pLacI + RBS + Cpn60 + RBS + Cpn10
• BBa_K538310: pBAD + RBS + Cpn10 + RBS + Cpn60
• BBa_K538311: pBAD + RBS + Cpn60 + RBS + Cpn10

The first two are controlled by the Lac operon's promoter, pLacI (BBa_R0010). The latter two are placed under control of a strongly arabinose-induced promoter, derived from pBAD by team [http://2010.igem.org/Team:British_Columba British Columbia 2009].

Refer to the main pages of this part's coding regions (Cpn10: BBa_K538000 and Cpn60: BBa_K538001) for more information on how their expression facilitates cold resistance in E. coli.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1419
  Illegal BamHI site found at 1485
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 278