Composite
Part:BBa_K515102:Experience
Designed by: Atipat Patharagulpong Group: iGEM11_Imperial_College_London (2011-09-07)
Characterisation
This part has been characterised qualitatively and quantitatively for chemotactic response towards L (-) malic acid. The tests were performed across a range of different attractant concentrations to observe optimal functioning of the part. Qualitative tests were performed using semi solid agar assays, quantitative analysis was performed using high - throughput capillary assay with cell count for each capillary evaluated using FACS. Behavioral analysis of E. coli expressing this part was done using Wide - field microscopy, and manual tracking was used to evaluate tactic response.
User Reviews
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