Device
eGFP-PTS1

Part:BBa_K678002:Design

Designed by: DTU-Denmark-2   Group: iGEM11_DTU-Denmark-2   (2011-09-02)
Revision as of 21:07, 10 September 2011 by Jaide (Talk | contribs) (→‎Design Notes)

eGFP-PTS1 for mammalian cells


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2233
    Illegal SpeI site found at 49
    Illegal PstI site found at 2323
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2233
    Illegal SpeI site found at 49
    Illegal PstI site found at 2323
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2233
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2233
    Illegal SpeI site found at 49
    Illegal PstI site found at 2323
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2233
    Illegal SpeI site found at 49
    Illegal PstI site found at 2323
    Illegal NgoMIV site found at 3088
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains the linker 1 at the 5'-end and the linker 6 at the 3'-end as shown on the figure below.

Overveiw_module_tailst2.png

Source

Source to be announced

References