Translational_Unit
Part:BBa_K515000
Designed by: Atipat Patharagulpong Group: iGEM11_Imperial_College_London (2011-09-01)
IaaM - tryptophan-2-mono-oxygenase
IaaM encodes IAA tryptophan monooxygenase which converts tryptophan to indoleacetamide. This is the first enzyme in a two-enzyme pathway from Psuedomonas savastanoi to produce indole 3 acetic acid
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 450
Illegal BamHI site found at 1395 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 157
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/enzyme
chassis | E. coli DH5α |
device_type | pathway |
input_s | tryptophan |
origin | P. savastanoi |
output | Indole acetamide |
resistance | chloramphenicol |