Reporter
mCherry

Part:BBa_J06504:Design

Designed by: ytwang   Group: iGEM2005   (2005-07-18)
Revision as of 19:25, 1 December 2006 by Scmohr (Talk | contribs) (→‎References)


monomeric RFP optimized for bacteria


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR.


Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.



Source

mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible

References

See main page.