Reporter

Part:BBa_I13522:Design

Designed by: jkm   Group: iGEM04_MIT   (2005-07-01)
Revision as of 03:47, 30 December 2010 by Acyang (Talk | contribs) (→‎Design Notes)

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pTet GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724


Design Notes

Primers: Note: EcoRI sites removed. Must use XP. Base pairs in CAPS are the truncated prefix and the suffix. Sequences provided by Gingko BioWorks.

Fwd: GCTTCTAGAGATACATGAAGATGCACTTtccctatcagtgatagagattgacatcc

Rv: CTGCAGCGGCCGCTACTAGTAAGGAGCGCTTtataaacgcagaaaggcccacccg

Source

References