Part:BBa_K648007

Medium 6AA Fusion Protein Linker: GGSGGS with Standard 25 Prefix/Suffix

This is the mid-sized linker of the three fusion protein linkers created by the Penn State 2011 iGEM team. It encodes for the six amino acids GGSGGS and has a prefix and suffix compatible with assembly standard 25. It is used in one of the variants of our Fast-Fusion protein reporter system.

This linker was cited in a very interesting article that talks about its high potential for use in "usefulness of tags and linker in plant molecular pharming", which can also be used in recombinant proteins. It was also cited in the American patent application for CAS9 FUSION MOLECULES, GENE EDITING SYSTEMS AND METHODS OF USING THEM (Request # 20190136210), as an additional linker, being a 6AA Medium Fusion Protein Linker.

Isoelectric Point: 5,52

Molecular Weight: 420,38Da

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

REFERENCES

1. Makhzoum, Abdullah & Tahir, Shifa & Locke, Majorie & Trémouillaux-Guiller, Jocelyne & Hefferon, Kathleen. (2014). An in silico overview on the usefulness of tags and linkers in plant molecular pharming. Plant Science Today. 1. 201-212. 10.14719/pst.2014.1.4.72.