Coding

Part:BBa_I20531

Designed by: Drew Endy   Group: Endy Lab   (2011-06-17)
Revision as of 23:32, 17 June 2011 by Drew (Talk | contribs) (→‎Property Rights)

CDS for phage k1-5 RNA polymerase

Coding sequence for K1-5 RNA polymerase. Useful for orthogonal transcription. Cognate consensus promoter encoded by BBa_I20530.

Property Rights

All rights not already held by others are PUBLIC DOMAIN as per [http://biobricks.org/bpa/contributions/2/ BPA Contributor Agreement #2] in compliance with [http://rph.stanford.edu/5-1.html Section 5.1.A.3] of the Stanford Research Policy Handbook.

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Acknowledgement

[http://www.biosci.utexas.edu/mgm/People/Faculty/profiles/?id=1707 Prof. Ian J. Molineux] of UT Austin suggested the K1-5 RNA polymerase system as a orthogonal transcription system distinct from T3, T7, SP6, et al.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 951
    Illegal SpeI site found at 658
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 658
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1467
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 951
    Illegal SpeI site found at 658
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 951
    Illegal SpeI site found at 658
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None