Coding

Part:BBa_I20531:Design

Designed by: Drew Endy   Group: Endy Lab   (2011-06-17)
Revision as of 17:54, 17 June 2011 by Drew (Talk | contribs) (→‎References)

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CDS for phage k1-5 RNA polymerase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 951
    Illegal SpeI site found at 658
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 658
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1467
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 951
    Illegal SpeI site found at 658
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 951
    Illegal SpeI site found at 658
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Native DNA sequence has not here been refined to support compatibility with any existing physical DNA assembly protocol.

Source

Nucleotide sequence of the annotated K1-5 genome as per Genbank AY370674.1

References

Genomic Analysis of Bacteriophages SP6 and K1-5, an Estranged Subgroup of the T7 Supergroup. Journal of Molecular Biology Volume 335, Issue 5, 30 January 2004, Pages 1151-1171 doi:10.1016/j.jmb.2003.11.035