DNA

Part:BBa_M12203:Design

Designed by: Grant Robinson   Group: MIT BE 20.20 - S09 MIT BE 20.20 - S10   (2011-01-07)
Revision as of 19:26, 12 January 2011 by Norville (Talk | contribs) (→‎Design Notes)

Amino Acids 176-240 of E0040


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 119


Design Notes

Not applicable. Forward primer: gtttcttcgaattcgcggccgcttctagaggttcaactagcagaccattatcaacaaaatac Forward primer: gtttcttcgaattcgcggccgcttctagagcaactagcagaccattatcaacaaaatactc Reverse primer: gtttcttcctgcagcggccgctactagtattattatttgtatagttcatccatgccatgtg Reverse primer: gtttcttcctgcagcggccgctactagtattattatttgtatagttcatccatgccatgtg


M12203f1: ttcttcgaattcgcggccgcttctagaggttcaactagcagaccattatcaacaaaatac

M12203f2: tttcttcgaattcgcggccgcttctagagcaactagcagaccattatcaacaaaatactc

M12203r: tttcttcctgcagcggccgctactagtattattatttgtatagttcatccatgccatgtg

Source

BBa_E0040.

References