Reporter

Part:BBa_K313007

Designed by: Mingshuo Zeng, Ryo Kariyazono, Ryo Taniuchi, Masahiko Shimizu, Kosuke Uekusa, Ryosuke Kamei   Group: iGEM10_UT-Tokyo   (2010-10-21)
Revision as of 20:55, 7 November 2010 by Kohaku (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

gfp generator in the reverse direction

This part is a gfp generator in reverse direction. It contains terminator. It is basically the reversed version of BBa_E0840.


verificatino experiment

The part is ligated with inverted pBAD promoter(BBa_J44002) and the plasmid was transformed into JM109.

Transcription of gfp generator (reverse) from the pBAD reverse promoter was induced by 10x10^(-2)M arabinose when OD_(600) reached 0.1 to 0.2. The result is shown below with the data of negative control that contains pBAD(reverse) and terminator.

  • The result of gfp expression assay


It is confirmed from the graph that this part properly worked.

Please see our presenteation slide and poster for our experiments utilized this part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 209


[edit]
Categories
Parameters
None