Reporter

Part:BBa_K313007

Designed by: Mingshuo Zeng, Ryo Kariyazono, Ryo Taniuchi, Masahiko Shimizu, Kosuke Uekusa, Ryosuke Kamei   Group: iGEM10_UT-Tokyo   (2010-10-21)
Revision as of 20:44, 7 November 2010 by Kohaku (Talk | contribs) (→‎verificatino experiment)

gfp generator in the reverse direction

This part is a gfp generator in reverse direction. It contains terminator. It is basically the reversed version of BBa_E0840.


verificatino experiment

The part is ligated with inverted pBAD promoter(BBa_K???) and the plasmid was transformed into JM109.


  • The result of gfp expression assay

Transcription of gfp generator (reverse) from the pBAD reverse promoter was induced by 10x10^(-2)M arabinose when OD_(600) reached 0.1 to 0.2.

Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LocSq Location sequence] assay page for detail.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 209


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