Reporter
lacZ a

Part:BBa_E0033

Designed by: Yong-Su Jin (Fighting Darwins)   Group: Antiquity   (2004-01-27)
Revision as of 14:38, 27 March 2007 by Scmohr (Talk | contribs)

LacZ alpha fragment; complements matching N-terminal deletion mutant (lacZ-omega)

To greatly reduce the number of bases, this is only a portion of the LacZ gene. It can be used as a detection system with N-terminal deletion mutants of lacZ. Combination of lacZ-alpha with such a deletion mutant protein restores enzyme activity that can be assayed colorimetrically or more sensitively with chemiluminescent substrates.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 93
    Illegal XhoI site found at 144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
//function/coliroid
//function/reporter
//function/reporter/color
Parameters
absvaried
emission
emit
excitation
excite
lumvaried
proteinLacZ-alpha
tagNone