Measurement

Part:BBa_K313017

Designed by: Ryosuke Kamei, Ryo Kariyazono   Group: iGEM10_UT-Tokyo   (2010-10-22)
Revision as of 19:32, 5 November 2010 by Kohaku (Talk | contribs)

terminator leakiness assay device

This is a device that aims to measure the leakiness of a terminator BBa_B1006.

If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part.

With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, you would be able to detect the quantity of Cre and assess the leakiness of the terminator.


verification experiment

This part is ligated with loxP-gfp-loxP sequence and transformed into E.coli. By incubating E.coli, then extracting plasmids, and sequencing them, it was proved that cre generated by BBa_K313008 is able to catalyze site specific recombination reaction.


Please see our [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page for detail.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 478
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 164
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None