Coding

Part:BBa_K339003:Design

Designed by: Emily Hicks   Group: iGEM10_Calgary   (2010-10-25)
Revision as of 00:32, 31 October 2010 by Emily Hicks (Talk | contribs)

malE31ΔSS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 360
    Illegal BamHI site found at 96
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 58


Design Notes

Obtained in plasmid form from the Betton labs in France (Betton et al., 2002). Used PCR to amplify the gene out of the plasmid and add the biobrick prefix and suffix.


Source

etton, J., Phichith, D. and Hunke, S. (2002). Folding and aggregation of export-defective mutants of the maltose-binding protein. Research in Microbiology. (153): 399-404.


References