Composite

Part:BBa_K329035

Designed by: Xavier Duportet   Group: iGEM10_Paris_Liliane_Bettencourt   (2010-10-25)
Revision as of 03:50, 5 November 2010 by Adecrulle (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

pBAD/araC - RBS - Xis_Int Tn916

This part carry the both enzyme Int and Xis of the conjugative transposon Tn916 under the control of the inducible promoter Pbad/AraC. The Tn916 is a "cut and paste" transposon which naturally move a 18kb DNA fragment carrying Tetracycline resistance. Tn916 is able to make intra and intercellular transfer thanks to a closed circular intermediate.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None