Coding

Part:BBa_K339000

Designed by: Emily Hicks   Group: iGEM10_Calgary   (2010-10-23)
Revision as of 23:20, 29 October 2010 by Emily Hicks (Talk | contribs)

Maltose Binding Protein (malE)

Maltose Binding Protein

A native protein to Escherichia Coli, malE is localized to the periplasm of the cell where it is able to fold extremely well.

Usage and Biology

malE forms part of the maltose/maltodextrin transport system in E. Coli. This system is responsible for the catabolism of malodextrins within the cell. Due to its ability to fold well, malE is also used as a fusion partner in order to improve the solubilities of many harder to express proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 435
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 133


Functional Parameters

[edit]
Categories
Parameters
None