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• 18:21, 15 September 2015 (diff | hist) . . (+3,660)‎ . . Part:BBa K1761002:Design ‎ (signaling components) (current)
• 18:13, 15 September 2015 (diff | hist) . . (-11)‎ . . Part:BBa K1761001:Design ‎ (figures) (current)
• 18:12, 15 September 2015 (diff | hist) . . (+417)‎ . . N File:TU Eindhoven RET Simplified Energy-level diagram.png ‎ (Simplified energy-level diagram of RET. A) Normally, an excited electron falls back to its ground state under the emission of light (a radiative transition). B) In the case of Resonance Electron Transfer, an excited electron in the donor falls back to its) (current)
• 18:01, 15 September 2015 (diff | hist) . . (+283)‎ . . N File:TU Eindhoven Efficient energy transfer.png ‎ (A) Efficient energy transfer is not possible since the donor and fluorophore are to remote. B) Efficient energy transfer is possible due to the proximity of the donor and acceptor. As a result, light intensity of the donor decreases whereas light intensit) (current)
• 17:57, 15 September 2015 (diff | hist) . . (+133)‎ . . N File:TU Eindhoven Resonance Energy Transfer and Bioluminiscence.png ‎ (Resonance energy transfer (A) and split luciferases (B) both translate close proximity into a measurable signal in the form of light.) (current)
• 16:46, 15 September 2015 (diff | hist) . . (+3,677)‎ . . Part:BBa K1761001:Design ‎
• 16:26, 15 September 2015 (diff | hist) . . (+25)‎ . . Part:BBa K1761000:Design ‎ (headings)
• 10:12, 15 September 2015 (diff | hist) . . (+6)‎ . . Part:BBa K1761000:Design ‎ (figures)
• 10:10, 15 September 2015 (diff | hist) . . (+6,570)‎ . . Part:BBa K1761000:Design ‎
• 10:09, 15 September 2015 (diff | hist) . . (+393)‎ . . N File:TU Eindhoven OmpX CPX.png ‎ (CPX (right) is a membrane protein derived from OmpX (left) through circular permutation. OmpX and CPX feature signal sequences (yellow) which ensure that the membrane protein is localized to E.coli’s outer membrane. These signal sequences are cleaved fr) (current)
• 10:04, 15 September 2015 (diff | hist) . . (+75)‎ . . N File:TU Eindhoven Tyrosine and Non-natural amino acid pAzF.png ‎ (The non-natural azide-functionalized amino acid closely resembles tyrosine.) (current)
• 10:02, 15 September 2015 (diff | hist) . . (+219)‎ . . N File:TU Eindhoven Click Chemistry CPX.png ‎ (The azide-functionalized amino acid enables clicking any DBCO-functionalized molecule to be attached to the cell membrane through SPAAC-click chemistry. iGEM TU Eindhoven 2014 called this azide-functionalized CPX COMPx.) (current)
• 10:02, 15 September 2015 (diff | hist) . . (+393)‎ . . N File:TU Eindhoven OmpX Clicked aptamer and signaling domain.png ‎ (CPX (right) is a membrane protein derived from OmpX (left) through circular permutation. OmpX and CPX feature signal sequences (yellow) which ensure that the membrane protein is localized to E.coli’s outer membrane. These signal sequences are cleaved fr) (current)
• 10:00, 15 September 2015 (diff | hist) . . (+437)‎ . . N File:TU Eindhoven Strain-Promoted Azide-Alkyne Cycloaddition.png ‎ (Strain-promoted Azide-Alkyne Cycloaddition (SPAAC) is a well known click-chemistry reaction. The reaction features two molecules, one functionalized with an azide group (A) and one functionalized with a cyclooctyne group (B). SPAAC is very efficient and s) (current)
• 08:12, 15 September 2015 (diff | hist) . . (+3)‎ . . Part:BBa K1761004 ‎ (References)
• 08:11, 15 September 2015 (diff | hist) . . (+3)‎ . . Part:BBa K1761002 ‎ (References)
• 08:10, 15 September 2015 (diff | hist) . . (+4)‎ . . Part:BBa K1761000 ‎ (References)
• 08:10, 15 September 2015 (diff | hist) . . (+3)‎ . . Part:BBa K1761001 ‎ (References)
• 08:09, 15 September 2015 (diff | hist) . . (+1,249)‎ . . Part:BBa K1761003 ‎ (adding schematical overviews)
• 14:57, 11 September 2015 (diff | hist) . . (+26)‎ . . N File:TU Eindhoven mNeonGreen 3D-structure.png ‎ (3D-structure of mNeonGreen) (current)
• 14:32, 11 September 2015 (diff | hist) . . (0)‎ . . File:TU Eindhoven OmpX NanoLuc SnapGene.png ‎ (uploaded a new version of "File:TU Eindhoven OmpX NanoLuc SnapGene.png": without background) (current)
• 14:31, 11 September 2015 (diff | hist) . . (0)‎ . . File:TU Eindhoven OmpX mNeonGreen SnapGene.png ‎ (uploaded a new version of "File:TU Eindhoven OmpX mNeonGreen SnapGene.png": without background) (current)
• 14:25, 11 September 2015 (diff | hist) . . (0)‎ . . File:TU Eindhoven OmpX SnapGene.png ‎ (uploaded a new version of "File:TU Eindhoven OmpX SnapGene.png") (current)
• 14:22, 11 September 2015 (diff | hist) . . (+434)‎ . . Part:BBa K1761002 ‎ (2x schematical overview + source)
• 14:16, 11 September 2015 (diff | hist) . . (+96)‎ . . N File:TU Eindhoven Construct OmpX NanoLuc.png ‎ (Schematical overview of the expressed OmpX (1) with NanoLuc (2) in the outer membrane of E.coli.)
• 13:12, 11 September 2015 (diff | hist) . . (+484)‎ . . Part:BBa K1761001 ‎ (→‎Fluorescence Confirmation: 2x fluorescence figure)
• 13:10, 11 September 2015 (diff | hist) . . (+258)‎ . . N File:TU Eindhoven Results Fluorescence mNeonGreen and NanoLuc.png ‎ (Fluorescence assay results of OmpX – mNeonGreen and OmpX - NanoLuc. Excitation of mNeonGreen took place at a wavelenght of 480 nm with a laser. Emission was read out at 517 nm. From this experiment, it can be concluded that mNeonGreen is present and wor) (current)
• 13:01, 11 September 2015 (diff | hist) . . (+240)‎ . . N File:TU Eindhoven Results Fluorescence mNeonGreen.png ‎ (Fluorescence assay results of OmpX – mNeonGreen. Excitation of mNeonGreen took place at a wavelenght of 480 nm with a laser. Emission was read out at 517 nm. From this experiment, it can be concluded that mNeonGreen is present and works.) (current)
• 12:39, 11 September 2015 (diff | hist) . . (+7)‎ . . Part:BBa K1761001 ‎ (→‎Gene Design)
• 11:35, 10 September 2015 (diff | hist) . . (+438)‎ . . Part:BBa K1761001 ‎ (adding schematical overviews)
• 11:32, 10 September 2015 (diff | hist) . . (+91)‎ . . N File:TU Eindhoven Construct OmpX mNeonGreen.png ‎ (Schematical overview of the expressed OmpX with mNeonGreen in the outer membrane of E.coli.)
• 11:17, 10 September 2015 (diff | hist) . . (+296)‎ . . Part:BBa K1761000 ‎ (adding picture of OmpX in membrane)
• 11:10, 10 September 2015 (diff | hist) . . (+166)‎ . . N File:TU Eindhoven Constructs OmpX NG and OmpX NL.png ‎ (Schematical overview of the expressed OmpX with mNeonGreen and OmpX with NanoLuc in the outer membrane of E.coli. The mNeonGreen and NanoLuc domains form a BRET pair.)
• 10:58, 10 September 2015 (diff | hist) . . (+351)‎ . . Part:BBa K1761002 ‎ (adding plasmid picture)
• 10:57, 10 September 2015 (diff | hist) . . (+260)‎ . . N File:TU Eindhoven OmpX NanoLuc SnapGene.png ‎ (Snapgene plasmid overview of the BioBrick part BBa_K1761002. It shows the pSB1C3 vector with the prefix (containing the restriction sites EcoRI and XbaI), wildtype OmpX, the BsoBI linker, NanoLuc and the suffix (containting the restiction sites SpeI and P)
• 10:31, 10 September 2015 (diff | hist) . . (+350)‎ . . Part:BBa K1761001 ‎ (adding plasmid picture)
• 10:31, 10 September 2015 (diff | hist) . . (+263)‎ . . N File:TU Eindhoven OmpX mNeonGreen SnapGene.png ‎ (Snapgene plasmid overview of the BioBrick part BBa_K1761001. It shows the pSB1C3 vector with the prefix (containing the restriction sites EcoRI and XbaI), wildtype OmpX, the BamHI linker, mNeonGreen and the suffix (containting the restiction sites SpeI an)
• 10:26, 10 September 2015 (diff | hist) . . (+312)‎ . . Part:BBa K1761000 ‎ (adding plasmid picture)
• 10:19, 10 September 2015 (diff | hist) . . (+14)‎ . . Part:BBa K1761000 ‎ (change picture size)
• 10:13, 10 September 2015 (diff | hist) . . (+233)‎ . . N File:TU Eindhoven OmpX SnapGene.png ‎ (Snapgene plasmid overview of the BioBrick part BBa_K1761000. It shows the pSB1C3 vector with the prefix (containing the restriction sites EcoRI and XbaI), wildtype OmpX and the suffix (containting the restiction sites SpeI and PstI).)
• 09:29, 10 September 2015 (diff | hist) . . (+625)‎ . . Part:BBa K1761004:Design ‎ (→‎References) (current)
• 09:16, 10 September 2015 (diff | hist) . . (+6)‎ . . Part:BBa K1761001 ‎ (change picture size)
• 21:59, 9 September 2015 (diff | hist) . . (+2,886)‎ . . Part:BBa K1761004 ‎ (Documentation)
• 21:44, 9 September 2015 (diff | hist) . . (+2,218)‎ . . Part:BBa K1761003 ‎ (Documentation, sources need to be added and fluorescence measurement)
• 21:27, 9 September 2015 (diff | hist) . . (+5,075)‎ . . Part:BBa K1761000 ‎ (Documentation, mTurquoise needs to get a link)
• 15:47, 9 September 2015 (diff | hist) . . (+4,777)‎ . . Part:BBa K1761002 ‎ (Documentation, bioluminiscence has to be added)
• 15:43, 9 September 2015 (diff | hist) . . (+584)‎ . . N File:TU Eindhoven FACS result OmpX NL.png ‎ (FACS result of OmpX - NanoLuc, after clicking with DBCO-PEG4-TAMRA. DBCO-PEG4-TAMRA Confirmation To confirm whether OmpX is in the membrane and whether or not the non-natural amino acid is being incorporated into OmpX, DBCO-PEG4-TAMRA was used. TAMRA is ) (current)
• 14:41, 9 September 2015 (diff | hist) . . (+5,028)‎ . . Part:BBa K1761001 ‎ (Broader description, fluorescence measurement still has to be done)
• 14:34, 9 September 2015 (diff | hist) . . (+587)‎ . . N File:TU Eindhoven FACS result OmpX NG.png ‎ (FACS result of OmpX - mNeonGreen, after clicking with DBCO-PEG4-TAMRA. DBCO-PEG4-TAMRA Confirmation To confirm whether OmpX is in the membrane and whether or not the non-natural amino acid is being incorporated into OmpX, DBCO-PEG4-TAMRA was used. TAMRA ) (current)
• 14:10, 9 September 2015 (diff | hist) . . (+490)‎ . . N File:TU Eindhoven design ompxstructure.png ‎ (A) The OmpX protein structure has been elucidated through NMR and X-ray crystallography, B) The square residues are important for the secondary structure of OmpX. To keep the structure intact, we introduce an amber stop codon in one of the protruding loop) (current)

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