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- 23:01, 3 October 2014 (diff | hist) . . (+4) . . Part:BBa K1352001
- 22:59, 3 October 2014 (diff | hist) . . (+311) . . Part:BBa K1352001
- 22:52, 3 October 2014 (diff | hist) . . (+31) . . Part:BBa K1352001
- 22:51, 3 October 2014 (diff | hist) . . (+23) . . Part:BBa K1352001
- 22:48, 3 October 2014 (diff | hist) . . (-4) . . Part:BBa K1352001
- 22:47, 3 October 2014 (diff | hist) . . (+8) . . Part:BBa K1352001
- 22:45, 3 October 2014 (diff | hist) . . (+3) . . Part:BBa K1352001
- 22:44, 3 October 2014 (diff | hist) . . (+4) . . Part:BBa K1352001
- 22:41, 3 October 2014 (diff | hist) . . (+26) . . Part:BBa K1352001
- 22:38, 3 October 2014 (diff | hist) . . (+266) . . Part:BBa K1352001
- 22:30, 3 October 2014 (diff | hist) . . (+8) . . Part:BBa K1352001
- 22:30, 3 October 2014 (diff | hist) . . (-5) . . Part:BBa K1352001
- 22:29, 3 October 2014 (diff | hist) . . (+174) . . Part:BBa K1352001
- 22:26, 3 October 2014 (diff | hist) . . (+85) . . Part:BBa K1352001
- 22:24, 3 October 2014 (diff | hist) . . (+12) . . Part:BBa K1352001
- 22:23, 3 October 2014 (diff | hist) . . (+29) . . Part:BBa K1352001
- 22:18, 3 October 2014 (diff | hist) . . (+186) . . m Part:BBa K1352001
- 22:15, 3 October 2014 (diff | hist) . . (-2) . . Part:BBa K1352001
- 22:14, 3 October 2014 (diff | hist) . . (+2) . . Part:BBa K1352001
- 22:13, 3 October 2014 (diff | hist) . . (+22) . . Part:BBa K1352001
- 22:01, 3 October 2014 (diff | hist) . . (+1,087) . . m Part:BBa K1352001
- 21:56, 3 October 2014 (diff | hist) . . (+8) . . m Part:BBa K1352001
- 21:55, 3 October 2014 (diff | hist) . . (+594) . . m Part:BBa K1352001
- 21:52, 3 October 2014 (diff | hist) . . (+186) . . Part:BBa K1352001
- 21:48, 3 October 2014 (diff | hist) . . (0) . . Part:BBa K1352001
- 21:47, 3 October 2014 (diff | hist) . . (0) . . m Part:BBa K1352001
- 21:46, 3 October 2014 (diff | hist) . . (-2) . . Part:BBa K1352001
- 21:45, 3 October 2014 (diff | hist) . . (+6) . . m Part:BBa K1352001
- 21:44, 3 October 2014 (diff | hist) . . (+17) . . Part:BBa K1352001
- 21:41, 3 October 2014 (diff | hist) . . (+22) . . m Part:BBa K1352001
- 21:39, 3 October 2014 (diff | hist) . . (+25) . . m Part:BBa K1352001
- 21:37, 3 October 2014 (diff | hist) . . (-14) . . m Part:BBa K1352001
- 21:36, 3 October 2014 (diff | hist) . . (+65) . . Part:BBa K1352001
- 21:34, 3 October 2014 (diff | hist) . . (+32) . . Part:BBa K1352001
- 21:29, 3 October 2014 (diff | hist) . . (+50) . . N File:K1352001 image2.png (Fig. 2 Plasmid map of created BioBrick construct.) (current)
- 21:28, 3 October 2014 (diff | hist) . . (+82) . . N File:K1352001 image3.png (Fig. 3 Restriction digest of created BioBrick with a range of restriction enzymes.) (current)
- 21:28, 3 October 2014 (diff | hist) . . (+172) . . N File:K1352001 image4.png (Fig. 4 Chromatogram obtained during the sequencing of created BioBrick. Important features highlighted: yellow - BglII cut site; green - FLAG tag; purple - HindIII cut site) (current)
- 21:27, 3 October 2014 (diff | hist) . . (+223) . . N File:K1352001 image5.png (Fig. 5 Translation of the theoretical construct and actual sequencing data. Query: Ag43-FLAG sequencing data; Subject - theoretical Ag43-FLAG construct. Pink box - FLAG tag translation flanked by BglII and HindIII cut sites) (current)
- 21:26, 3 October 2014 (diff | hist) . . (+171) . . N File:K1352001 image6.png (Fig. 6 Western blot of a total cell lysate of an E. coli Bba_K1352001 transformant, run on a 12% SDS-PAGE gel, blotted onto membrane and probed with an anti-FLAG antibody.) (current)
- 21:26, 3 October 2014 (diff | hist) . . (+66) . . N File:K1352001 image7.png (Fig. 7 Schematic representation of immunolabelling technique used.) (current)
- 21:25, 3 October 2014 (diff | hist) . . (+110) . . N File:K1352001 image8.png (Fig. 8 Results of the immunolabelling of created construct. A) red filter, B) brightfield, C) composite image.) (current)
- 21:25, 3 October 2014 (diff | hist) . . (+94) . . N File:K1352001 image9.png (Fig. 9 Imunolabelling of Bba_K1352000 (negative control). A) red filter, B) brightfield image.) (current)
- 21:22, 3 October 2014 (diff | hist) . . (+140) . . N File:K1352001 image10.png (Fig. 10 Aggregation of Ag43 without FLAG (Bba_K1352000) (left) and Ag43+FLAG+MCS (Bba_K1352001) (right) after induction with 0.2% arabinose.) (current)
- 22:02, 2 October 2014 (diff | hist) . . (0) . . m Part:BBa K1352012 (current)
- 22:01, 2 October 2014 (diff | hist) . . (+2,965) . . N Part:BBa K1352012 (Created page with "Antigen 43 (sometimes called Ag43 or fluffing protein) is a phase-variable outer membrane protein encoded by flu gene. It is native to E.Coli K12 strain and is usually expressed ...")
- 21:20, 2 October 2014 (diff | hist) . . (+8,874) . . N Part:BBa K1352001 (Created page with "'''Abstract''' We describe the creation of Biobrick constrct Bba_K1352001 which expresses autotransporter Ag43 with an inserted FLAG epitope tag at codon 148, flanked by multiple...")
- 21:00, 2 October 2014 (diff | hist) . . (+1,107) . . Part:BBa K1352011
- 21:00, 2 October 2014 (diff | hist) . . (-1,107) . . Part:BBa K1352011
- 20:55, 2 October 2014 (diff | hist) . . (+1) . . m Part:BBa K1352011
- 20:49, 2 October 2014 (diff | hist) . . (+1,965) . . N Part:BBa K1352011 (Created page with "Antigen 43 (sometimes called Ag43 or fluffing protein) is a phase-variable outer membrane protein encoded by flu gene. It is native to E.Coli K12 strain and is usually expressed ...")
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