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File list
 
Date Name Thumbnail Size Description Versions
01:28, 18 October 2014 Png.png (file) 113 KB   1
19:15, 12 October 2014 Background.png (file) 105 KB   1
17:43, 12 October 2014 Beta 12.png (file) 217 KB 12 1
17:42, 12 October 2014 Beta 11.png (file) 258 KB 11 1
17:36, 12 October 2014 Beta 10.png (file) 173 KB 10 1
17:35, 12 October 2014 Beta 9.png (file) 16 KB 9 1
17:35, 12 October 2014 Beta 8.png (file) 113 KB 8 1
17:35, 12 October 2014 Beta 7.png (file) 128 KB 7 1
17:35, 12 October 2014 Beta 6.png (file) 254 KB 6 1
17:34, 12 October 2014 Beta 5.png (file) 162 KB 5 1
17:34, 12 October 2014 Beta 4.png (file) 9 KB 4 1
17:33, 12 October 2014 Beta 3.png (file) 100 KB 3 1
17:33, 12 October 2014 Beta 2.png (file) 103 KB 2 1
23:49, 3 October 2014 K1352001 image16.png (file) 37 KB 15 1
23:47, 3 October 2014 K1352001 image14.png (file) 25 KB 14 1
23:43, 3 October 2014 K1352001 image13.png (file) 346 KB 13 1
23:43, 3 October 2014 K1352001 image12.png (file) 296 KB 12 1
23:12, 3 October 2014 K1352001 image11.png (file) 102 KB 11 1
21:29, 3 October 2014 K1352001 image2.png (file) 104 KB Fig. 2 Plasmid map of created BioBrick construct. 1
21:28, 3 October 2014 K1352001 image3.png (file) 423 KB Fig. 3 Restriction digest of created BioBrick with a range of restriction enzymes. 1
21:28, 3 October 2014 K1352001 image4.png (file) 46 KB Fig. 4 Chromatogram obtained during the sequencing of created BioBrick. Important features highlighted: yellow - BglII cut site; green - FLAG tag; purple - HindIII cut site 1
21:27, 3 October 2014 K1352001 image5.png (file) 30 KB Fig. 5 Translation of the theoretical construct and actual sequencing data. Query: Ag43-FLAG sequencing data; Subject - theoretical Ag43-FLAG construct. Pink box - FLAG tag translation flanked by BglII and HindIII cut sites 1
21:26, 3 October 2014 K1352001 image6.png (file) 59 KB Fig. 6 Western blot of a total cell lysate of an E. coli Bba_K1352001 transformant, run on a 12% SDS-PAGE gel, blotted onto membrane and probed with an anti-FLAG antibody. 1
21:26, 3 October 2014 K1352001 image7.png (file) 7 KB Fig. 7 Schematic representation of immunolabelling technique used. 1
21:25, 3 October 2014 K1352001 image8.png (file) 298 KB Fig. 8 Results of the immunolabelling of created construct. A) red filter, B) brightfield, C) composite image. 1
21:25, 3 October 2014 K1352001 image9.png (file) 699 KB Fig. 9 Imunolabelling of Bba_K1352000 (negative control). A) red filter, B) brightfield image. 1
21:22, 3 October 2014 K1352001 image10.png (file) 297 KB Fig. 10 Aggregation of Ag43 without FLAG (Bba_K1352000) (left) and Ag43+FLAG+MCS (Bba_K1352001) (right) after induction with 0.2% arabinose. 1