Difference between revisions of "Part:BBa K316001"
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Aims of experiment | Measuring the activity of BioBrick promoters using an in vivo reference standard. | Aims of experiment | Measuring the activity of BioBrick promoters using an in vivo reference standard. | ||
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See Experience page for more information | See Experience page for more information | ||
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For more information about our project please visit our wiki[http://2010.igem.org/Team:Imperial_College_London] | For more information about our project please visit our wiki[http://2010.igem.org/Team:Imperial_College_London] | ||
Revision as of 00:53, 28 October 2010
pVeg Constitutive promoter for Veg locus from B. subtilis
This part is identical to the sequence submitted by Imperial 2008 team, this part was produced from BBa_K143053 by PCR. PVeg is a constitutive promoter controlled by Sigma factor A. This promoter has two binding sites which leads to high expression of downstream genes. There is some evidence that the sporulation master regulator the spoOA can interact with pVeg although the mechanism is not known.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization in r.p.u. of Pveg promoter
Aims of experiment | Measuring the activity of BioBrick promoters using an in vivo reference standard.
Results | pVeg promoter in pSB1C3 vector, a high copy plasmid, has an 1.62 r.p.u value and in 3K3 vector, a low copy plasmid an 0.79 r.p.u. value. These values were derived by dividing signal from the production of HMS by the pVeg promoter population of cells by signal from the standard promoter J23101 (r.p.u value of 1).
See Experience page for more information
For more information about our project please visit our wiki[http://2010.igem.org/Team:Imperial_College_London]