Difference between revisions of "Part:BBa K316004"

Revision as of 00:48, 28 October 2010

Functional XylE under J23101 promoter, with double terminator

Standard constitutive E.coli promoter BBa_J23101 combined with BBa_K316003 for comparative characterisation of gene activity (catechol breakdown into 2,4 hydromuconic semialdehyde yellow product).

For more information about XylE, it's substrate and spectrophotometric assays, please see BBa_K316003 or our wiki[http://2010.igem.org/Team:Imperial_College_London/Results]

Safety

The substrate XylE works on is a chemical called catechol. It is classed as irritant in the EU but as toxic in the USA, as well as being a possible carcinogen. It should therefore be handled with care and proper safety equipment. More information is available on the Material Safety Data Sheet[http://www.sciencelab.com/msds.php?msdsId=9927131].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 380
Illegal NgoMIV site found at 552
Illegal AgeI site found at 903
1000
COMPATIBLE WITH RFC[1000]

Part Characterisation

Characterisation data was obtained for XylE BBa_K316003. In addition constructs under two different promoters: J23101-XylE BBa_K316004 from E. coli was used to categorise B. subtilis derived Pveg-XylE BBa_K316005. Also GFP-XylE constructs BBa_K316007 were tested to determine the effectiveness of inhibition of XylE activity by attachment of GFP. These are described on our wiki[http://2010.igem.org/Team:Imperial_College_London/Results] and the aforementioned parts pages.

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K316004 short</partinfo>
-Standard constitutive E.coli promoter <bbpart>BBa_J23101</bbpart> combined with <bbpart>BBa_K316003</bbpart> for comparative characterisation of gene activity (catechol breakdown into 2,4 hydroxyaminobutane yellow product).
+Standard constitutive E.coli promoter <bbpart>BBa_J23101</bbpart> combined with <bbpart>BBa_K316003</bbpart> for comparative characterisation of gene activity (catechol breakdown into 2,4 hydromuconic semialdehyde yellow product).
 For more information about XylE, it's substrate and spectrophotometric assays, please see <bbpart>BBa_K316003</bbpart> or our wiki[http://2010.igem.org/Team:Imperial_College_London/Results]