Difference between revisions of "Part:BBa K316001"

Revision as of 00:18, 28 October 2010

pVeg Constitutive promoter for Veg locus from B. subtilis

This part is identical to the sequence submitted by Imperial 2008 team, this part was produced from BBa_K143053 by PCR. PVeg is a constitutive promoter controlled by Sigma factor A. This promoter has two binding sites which leads to high expression of downstream genes. There is some evidence that the sporulation master regulator the spoOA can interact with pVeg although the mechanism is not known.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Characterization in r.p.u. of Pveg promoter

Aims of experiment | Measuring the activity of BioBrick promoters using an in vivo reference standard.

Results | pVeg promoter in pSB1C3 vector, a high copy plasmid, has an 1.62 r.p.u value and in 3K3 vector, a low copy plasmid an 0.79 r.p.u. value. These values were derived by dividing signal from the production of HMS by the pVeg promoter population of cells by signal from the standard promoter J23101 (r.p.u value of 1).

For more information about our project please visit our wiki[http://2010.igem.org/Team:Imperial_College_London]

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+Characterization in r.p.u. of Pveg promoter
+Aims of experiment | Measuring the activity of BioBrick promoters using an in vivo reference standard.
+Results | pVeg promoter in pSB1C3 vector, a high copy plasmid, has an 1.62 r.p.u value and in 3K3 vector, a low copy plasmid an 0.79 r.p.u. value. These values were derived by dividing signal from the production of HMS by the pVeg promoter population of cells by signal from the standard promoter J23101 (r.p.u value of 1).
 ----
 For more information about our project please visit our wiki[http://2010.igem.org/Team:Imperial_College_London]