Difference between revisions of "Part:BBa K316005"
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<span class='h3bb'><big>'''Part Characterisation'''</big></span>
 
<span class='h3bb'><big>'''Part Characterisation'''</big></span>
  
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Characterisation data was obtained using GFP-XylE constructs <bbpart>BBa_K316007</bbpart> and XylE under two different promoters: ''B. subtilis'' derived Pveg <bbpart>BBa_K316005</bbpart> and J23101 <bbpart>BBa_K316004</bbpart> from ''E. coli''. These are described on our wiki[http://2010.igem.org/Team:Imperial_College_London/Results] and the aforementioned parts pages.
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Characterisation data was obtained for XylE <bbpart>BBa_K316003</bbpart>. In addition constructs under two different promoters: J23101-XylE <bbpart>BBa_K316004</bbpart> from ''E. coli'' was used to categorise ''B. subtilis'' derived Pveg-XylE <bbpart>BBa_K316005</bbpart>. Also GFP-XylE constructs <bbpart>BBa_K316007</bbpart> were tested to determine the effectiveness of repression. These are described on our wiki[http://2010.igem.org/Team:Imperial_College_London/Results] and the aforementioned parts pages.

Revision as of 23:42, 27 October 2010

Functional XylE under Pveg promoter, with double terminator

Enables characterization and comparison with alternative promoters e.g. BBa_J23101 Designed for characterisation of XylE in Bacillus subtilis where Pveg is a useful constitutive promoter.

For more information about XylE, it's substrate and spectrophotometric assays, please see BBa_K316003 or our wiki[http://2010.igem.org/Team:Imperial_College_London/Results]


Safety

The substrate XylE works on is a chemical called catechol. It is classed as irritant in the EU but as toxic in the USA, as well as being a possible carcinogen. It should therefore be handled with care and proper safety equipment. More information is available on the Material Safety Data Sheet[http://www.sciencelab.com/msds.php?msdsId=9927131].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 442
    Illegal NgoMIV site found at 614
    Illegal AgeI site found at 965
  • 1000
    COMPATIBLE WITH RFC[1000]


Part Characterisation

Characterisation data was obtained for XylE BBa_K316003. In addition constructs under two different promoters: J23101-XylE BBa_K316004 from E. coli was used to categorise B. subtilis derived Pveg-XylE BBa_K316005. Also GFP-XylE constructs BBa_K316007 were tested to determine the effectiveness of repression. These are described on our wiki[http://2010.igem.org/Team:Imperial_College_London/Results] and the aforementioned parts pages.