Difference between revisions of "Part:BBa K299808"

Revision as of 23:19, 27 October 2010

This part is licensed under
Creative BioCommons

minC under pT7 and B0034

Construct for testing MinC cell division inhibitor protein.

It consists of the following parts: promoter T7 BBa_I719005 + BBa_B0034 RBS + MinC BBa_K299806.

This construct was generating no transformants in BL21 strain in no induction conditions. This was probably due to leaky expression of MinC from pT7 that resulted in constitutive growth inhibition. However we were able to work with it in E. coli Top10 to confirm it's correctness.

This construct was used for [http://2010.igem.org/Team:Warsaw/Stage2/Results MinC performance measurements].

Visit our [http://2010.igem.org/Team:Warsaw/Stage2/Results wiki] and [http://2010.igem.org/Team:Warsaw/Stage2/Design] for more detailed comments on the results and more.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 701
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 701
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 701
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 701
Illegal AgeI site found at 260
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 Construct for testing [https://parts.igem.org/wiki/index.php?title=Part:BBa_K299806 MinC cell division inhibitor protein].
 <br>
-It consists of the following parts: promoter T7 <partinfo>BBa_I719005</partinfo> + <partinfo>BBa_B0034</partinfo> RBS + MinC <partinfo>BBa_K299806</partinfo>.
+<p>It consists of the following parts: promoter T7 <partinfo>BBa_I719005</partinfo> + <partinfo>BBa_B0034</partinfo> RBS + MinC <partinfo>BBa_K299806</partinfo>. </p>
+<br>
 This construct was generating no transformants in BL21 strain in no induction conditions. This was probably due to leaky expression of MinC from pT7 that resulted in constitutive growth inhibition. However we were able to work with it in E. coli Top10 to confirm it's correctness.