Difference between revisions of "Part:BBa K316021"
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For reliable expression of spectinomycin resistance gene (Aad9 <bbpart>BBa_K143031</bbpart>)  in B. subtilis, the strongest constitutive promoter Pveg and strong ribosome binding site spoVG <bbpart>BBa_K143053</bbpart> were used. A double terminator <bbpart>BBa_B0015</bbpart> ensures there is minimal read-through.
 
For reliable expression of spectinomycin resistance gene (Aad9 <bbpart>BBa_K143031</bbpart>)  in B. subtilis, the strongest constitutive promoter Pveg and strong ribosome binding site spoVG <bbpart>BBa_K143053</bbpart> were used. A double terminator <bbpart>BBa_B0015</bbpart> ensures there is minimal read-through.
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[[Image:SpecCasette.PNG|center|800px]]
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'''Figure I.''' Spectinomycin resistance cassette.
  
  

Revision as of 18:25, 27 October 2010

Spectinomycin resistance with promoter for B.subtilis

For reliable expression of spectinomycin resistance gene (Aad9 BBa_K143031) in B. subtilis, the strongest constitutive promoter Pveg and strong ribosome binding site spoVG BBa_K143053 were used. A double terminator BBa_B0015 ensures there is minimal read-through.


SpecCasette.PNG

Figure I. Spectinomycin resistance cassette.


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 611