Difference between revisions of "Part:BBa K332011:Design"
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To make cry11 gene to be a iGEM standard part, we clone the cry11Aa gene from Bacillus thuringiensis subsp. Israelensis into TA vector. Besides, we process point mutations on the cry11Aa gene to remove two of the enzyme sites(EcoRI and SpeI) respectively. Furthermore, we design the primer to add the restriction enzyme sites EcoRI,XbarI,SpeI and PstI on the ends of cry11Aa gene by PCR and then transfer this fragment into psb1C3 backbone. | To make cry11 gene to be a iGEM standard part, we clone the cry11Aa gene from Bacillus thuringiensis subsp. Israelensis into TA vector. Besides, we process point mutations on the cry11Aa gene to remove two of the enzyme sites(EcoRI and SpeI) respectively. Furthermore, we design the primer to add the restriction enzyme sites EcoRI,XbarI,SpeI and PstI on the ends of cry11Aa gene by PCR and then transfer this fragment into psb1C3 backbone. | ||
| − | [[Image:cry11Aa(EXSP)]] | + | [[Image:cry11Aa(EXSP).jpg]] |
In our 2010 NCTU_Formosa project, the cry11Aa gene is the main part of cry weapon system and is regulated by temperature control system(BBa_K332031)[https://parts.igem.org/Part:BBa_K332031:Design]. | In our 2010 NCTU_Formosa project, the cry11Aa gene is the main part of cry weapon system and is regulated by temperature control system(BBa_K332031)[https://parts.igem.org/Part:BBa_K332031:Design]. | ||
Revision as of 13:29, 27 October 2010
Cry11Aa is one of the crystal proteins coded in Bacillus thuringiensis subsp. Israelensis. It is highly toxic to certain dipteran larvae, such as Aedes, and Anopheles larvae. The length of Cry11Aa sequence is about 1.9Kb.
To make cry11 gene to be a iGEM standard part, we clone the cry11Aa gene from Bacillus thuringiensis subsp. Israelensis into TA vector. Besides, we process point mutations on the cry11Aa gene to remove two of the enzyme sites(EcoRI and SpeI) respectively. Furthermore, we design the primer to add the restriction enzyme sites EcoRI,XbarI,SpeI and PstI on the ends of cry11Aa gene by PCR and then transfer this fragment into psb1C3 backbone.
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In our 2010 NCTU_Formosa project, the cry11Aa gene is the main part of cry weapon system and is regulated by temperature control system(BBa_K332031)[1].