Part:BBa_K332031:Design
Constitutive promoter + 37℃ induced RBS + tetR + double terminator
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 794
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To regulate following genes by temperature.
To maintain cells at 37° C ↑ : the tetR protein be generated and repress Ptet's circuit.
To release cells at 37° C ↓ : the tetR protein not be generated and do not repress Ptet's circuit.
The theoretical switching temperature of BBa_k115002,
which was designed by Team TUDelft in 2008, is 37℃.
Thus, our thermosensing device A can be induced at this temperature. (The switching temperature can be modified by changing RBS according to different conditions.)
Once being swiched on, tetR will inhibit the corresponding promoter, pTet, which enables us to regulate the expression of cry gene and quorum sensing gene devices.
sequence result: ATAATTCTGACTTAGCTATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGA TGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTTACAGCTAGCTCAGTCCTAGGTATTATGCTAGCTACTAGAGGGACAA GCAATGCTTGCCTTGAATAGTAACTTTTGAATAGTGATTCAGGAGGTACTAGATGTCCAGATTAGATAAAAGTAAAGTGA TTAACAGCGCATTAGAGCTGCTTAATGAGGTCGGAATCGAAGGTTTAACAACCCGTAAACTCGCCCAGAAGCTAGGTGTA GAGCAGCCTACATTGTATTGGCATGTAAAAAATAAGCGGGCTTTGCTCGACGCCTTAGCCATTGAGATGTTAGATAGGCA CCATACTCACTTTTGCCCTTTAGAAGGGGAAAGCTGGCAAGATTTTTTACGTAATAACGCTAAAAGTTTTAGATGTGCTT TACTAAGTCATCGCGATGGAGCAAAAGTACATTTAGGTACACGGCCTACAGAAAAACAGTATGAAACTCTCGAAAATCAA TTAGCCTTTTTATGCCAACAAGGTTTTTCACTAGAGAATGCATTATATGCACTCAGCGCTGTGGGGCATTTTACTTTAGG TTGCGTATTGGAAGATCAAGAGCATCAAGTCGCTAAAGAAGAAAGGGAAACACCTACTACTGATAGTATGCCGCCATTAT TACGACAAGCTATCGAATTATTTGATCACCAAGGTGCAGAGCCAGCCTTCTTATTCGGCCTTGAATTGATCATATGCGGA TTAGAAAAACAACTTAAATGTGAAAGTGGGTCCGCTGCAAACGACGAAAACTACGCTTTAGTAGCTTAATAACACTGATA GTGCTAGTGTAGATCACTACTAGAGCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTT TTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAATACTAGTCTGCAGTCCGGCAAA AAAGGGCAAGGTGTCACCACCCCTGCCCTTTTTCTTTAAAACCGAAAAAGATTACTTCGCGTTATGCAGCTCCTCGCTCA CTGACTCGCTGCGCTCGTCGTCGCCTGCGGCGAGCCGTATCAGCTCCACTCAAAGCGTATACGCTTATTCCCAGAATCAG GCATACGCAGATAAGAACATGTGAGCCAAGGCCAGCTAAAAGGCCAGGTAACCGA
match IGME's database
Source
the 37℃ induced RBS is from iGEM08_TUDelft