Difference between revisions of "Part:BBa K316011"

Revision as of 10:31, 27 October 2010

Reverse strand coding XylE

This part contains XylE coding on the 3' strand. In many biological settings there may be read-through caused by upstream elements, however this can often be unidirectional. In settings where readthrough from 5' direction is expected to be much stronger than 3' direction, it may is advantageous to use 3' coding sequence instead of traditional 5'.

For the purposes of characterisation, the part was made on the minus strand to reduce background transcription. Read-through from 5' direction may provide an additional benefit. Low quantities of anti-sence RNA may be produced as the result of 5' readthrough, which may in turn reduce basal expression sometimes seen in LacI systems.

For more information about XylE, it's substrate and spectrophotometric assays, please see BBa_K316003 or our wiki[http://2010.igem.org/Team:Imperial_College_London/Results]

Safety

The substrate XylE works on is a chemical called catechol. It is classed as irritant in the EU but as toxic in the USA, as well as being a possible carcinogen. It should therefore be handled with care and proper safety equipment. More information is available on the Material Safety Data Sheet[http://www.sciencelab.com/msds.php?msdsId=9927131].

Sequence and Features

Characterisation data was obtained using GFP-XylE constructs BBa_K316008 and XylE under two different promoters: B. subtilis derived Pveg BBa_K316005 and J23101 BBa_K316004 from E. coli. These are described on our wiki[http://2010.igem.org/Team:Imperial_College_London/Results] and the aforementioned parts pages.