Difference between revisions of "Part:BBa K329005"
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<partinfo>BBa_K329005 short</partinfo> | <partinfo>BBa_K329005 short</partinfo> | ||
| − | LacZ-alpha fragment (<partinfo>BBa_I732006</partinfo>) with a Strong RBS upstream (<partinfo>BBa_B0034</partinfo>). In strains with LacZ-omega (LacZ N-terminal deletion mutants) like DH5alpha, DH10B and Top10, LacZ-alpha restores the beta-galactosidase activity. | + | LacZ-alpha fragment (<partinfo>BBa_I732006</partinfo>) with a Strong RBS upstream (<partinfo>BBa_B0034</partinfo>). In strains with LacZ-omega (LacZ N-terminal deletion mutants) like DH5alpha, DH10B and Top10, LacZ-alpha restores the beta-galactosidase activity. In presence of Xgal, cells become blue. |
We have also designed the same construct with a Weak RBS (<partinfo>BBa_K329006</partinfo>). | We have also designed the same construct with a Weak RBS (<partinfo>BBa_K329006</partinfo>). | ||
Latest revision as of 16:37, 26 October 2010
Strong RBS (B0034) - LacZ-alpha fragment (I732006)
LacZ-alpha fragment (BBa_I732006) with a Strong RBS upstream (BBa_B0034). In strains with LacZ-omega (LacZ N-terminal deletion mutants) like DH5alpha, DH10B and Top10, LacZ-alpha restores the beta-galactosidase activity. In presence of Xgal, cells become blue.
We have also designed the same construct with a Weak RBS (BBa_K329006).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]