Difference between revisions of "Part:BBa K411201:Design"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K411201 short</partinfo>
 
<partinfo>BBa_K411201 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
without Lva tag the normal CFP will not have the speedy degradation.  
+
Originated from ''A. victoria'' GFP. Has the same coding region as [[Part:BBa_E0021]].
−
It's half-live are much longer
+
We wanted a version of the ECFP that did not have an LVA tag.
−
 
+
  
  
 
===Source===
 
===Source===
 +
PCR of [[Part:BBa_E0022]] to remove the LVA tag. Primers used (our [http://2010.igem.org/Team:NYMU-Taipei/ "xFP LVA remover"]):
 +
*: 5' -
 +
*: 5' -
  
−
biobrick BBa-E0022
 
  
 
===References===
 
===References===

Revision as of 15:04, 26 October 2010

Cyan Fluorescent Protein (CFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Originated from A. victoria GFP. Has the same coding region as Part:BBa_E0021. We wanted a version of the ECFP that did not have an LVA tag.


Source

PCR of Part:BBa_E0022 to remove the LVA tag. Primers used (our [http://2010.igem.org/Team:NYMU-Taipei/ "xFP LVA remover"]):

  • 5' -
    5' -


References