Difference between revisions of "Part:BBa K325909"
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'''Performance'''<br>
 
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<center>
 
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{|{{Table}}
 
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!Experiment<sup>1</sup>
 
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!Characteristic<sup>1</sup>
 
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!Value<sup>1</sup>
 
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|-
 
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|rowspan="3"|[[Part:BBa_F2620:Transfer Function|'''Transfer Function''']]
 
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|''Maximum Output''
 
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|6.6 [[PoPS]] cell<sup>-1</sup>
 
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|-
 
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|''Hill coefficient''
 
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|1.6
 
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|-
 
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|[[Switch Point|''Switch Point'']]
 
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|1.5E-9 M [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous
 
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|-
 
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|[[Part:BBa_F2620:Response time|'''Response time:''']]
 
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|<1 min
 
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|-
 
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|rowspan="2"|[[Part:BBa_F2620:Specificity|'''Input compatibility''']]
 
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|''Strong response to''
 
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|[[3OC6HSL|3OC<sub>6</sub>HSL]], C<sub>6</sub>HSL , C<sub>7</sub>HSL, 3OC<sub>8</sub>HSL, C<sub>8</sub>HSL
 
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|-
 
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|''Weak response to''
 
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|C<sub>4</sub>HSL, C<sub>10</sub>HSL, C<sub>12</sub>HSL
 
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|-
 
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|rowspan="2"|[[Part:BBa_F2620:Stability|'''Stability''']]
 
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|[[Genetic Stability|''Genetic Stability'']]<br>(Low/High Input)
 
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|>92/>56 generations
 
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|-
 
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|[[Performance Stability|''Performance Stability'']]<br>(Low/High Input)
 
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|>92/>56 generations
 
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|-
 
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|rowspan="4"|Demand
 
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|rowspan="1"|Internal Demand<br>(Low/High Input)
 
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|Not measured
 
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|-
 
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|rowspan="2"|[[Transcription Demand|''Transcriptional output demand:'']]<br>(Low/High Input)<br>Nt = length of downstream transcript in nucleotides
 
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|(0/6xNt) nucleotides cell<sup>-1</sup> s<sup>-1</sup>
 
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|-
 
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|(0/1.5E-1xNt) RNAP cell<sup>-1</sup>
 
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|-
 
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|[[Growth Rate|''Growth Rate'']]<br>(Low/High Input)
 
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|54/59 min Doubling time
 
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|}
 
 
</center>
 
</center>
 
<sup>1</sup>Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]
 
<sup>1</sup>Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]

Revision as of 22:32, 25 October 2010

Input: L-Arabinose
Output: Light

pBad/araC
I0500		PoPS to Light
 Cambridge-Eglowli.png

Part Main Page        Arabinose -> Light        H-NS mutants        Add Data       


Description
G28 !

THIS PAGE IS CURRENTLY BEING UPDATED.


</center> 1Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]

Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3


References
[http://www.jstor.org/stable/4449975 [1]:] J. Slock, (1995) Transformation Experiments Using Bioluminescence Genes of Vibrio fischeri,The American Biology Teacher, 57, 225-227.

[http://www.annualreviews.org/doi/pdf/10.1146/annurev.mi.42.100188.001055 [2]:] E.A. Meighen (1988) Enzymes and genes from the lux operons of bioluminescent bacteria, Annual Reviews in Microbiology 42, 151-176.

[http://www.annualreviews.org/doi/pdf/10.1146/annurev.ge.28.120194.001001 [3]:] E.A. Meighen, (1994) Genetics of bacterial bioluminescence, Annual Reviews of Genetics, 28, 117-139.