Difference between revisions of "Part:BBa K342002:Design"
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===Design Notes=== | ===Design Notes=== | ||
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− | <style=font-family: | + | <style="font-family:Arial Black;"><p>This part is a self cleavable fusion protein. It has been designed thanks to the results of the publication |
<a href="http://2010.igem.org/Team:INSA-Lyon/Project/References#et1"><font color= black><i>Banki et al.</i><font> | <a href="http://2010.igem.org/Team:INSA-Lyon/Project/References#et1"><font color= black><i>Banki et al.</i><font> | ||
</a>The phasin protein bind to the granule membrane. The second phasin makes the binding stronger and then increases the rate of purified protein during the step of granules extraction. The intein is a self cleavable protein which breaks under PH=6 and allows the release od the fused protein at the end of the sequence</p> | </a>The phasin protein bind to the granule membrane. The second phasin makes the binding stronger and then increases the rate of purified protein during the step of granules extraction. The intein is a self cleavable protein which breaks under PH=6 and allows the release od the fused protein at the end of the sequence</p> |
Revision as of 15:09, 25 October 2010
Phasin-Phasin Intein (for silver standard fusion)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1693
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1279
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Source
Ligation of two synthetized parts : Phasin and Phasin-Intein