Difference between revisions of "Part:BBa K342002:Design"

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===Design Notes===
 
===Design Notes===
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<html><p>This part is a self cleavable fusion protein. It has been designed thanks to the results of the publication
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<style="font-family:serif;"><p>This part is a self cleavable fusion protein. It has been designed thanks to the results of the publication
 
  <a href="http://2010.igem.org/Team:INSA-Lyon/Project/References#et1"><font color= black><i>Banki et al.</i><font>
 
  <a href="http://2010.igem.org/Team:INSA-Lyon/Project/References#et1"><font color= black><i>Banki et al.</i><font>
 
</a>The phasin protein bind to the granule membrane. The second phasin makes the binding stronger and then increases the rate of purified protein during the step of granules extraction. The intein is a self cleavable protein which breaks under PH=6 and allows the release od the fused protein at the end of the sequence</p>
 
</a>The phasin protein bind to the granule membrane. The second phasin makes the binding stronger and then increases the rate of purified protein during the step of granules extraction. The intein is a self cleavable protein which breaks under PH=6 and allows the release od the fused protein at the end of the sequence</p>
 
<p><br>The prefix biobrick standard contains a perfect RBS:, </br>The suffix is a silver standard fusion suffix :The phasin sequence is the same as the biobrick <a href="https://parts.igem.org/Part:BBa_K208001"><font color= "black"><i><u>BBa_K208001</u></i></font></a> from the Utah 2009 team. The sequence  
 
<p><br>The prefix biobrick standard contains a perfect RBS:, </br>The suffix is a silver standard fusion suffix :The phasin sequence is the same as the biobrick <a href="https://parts.igem.org/Part:BBa_K208001"><font color= "black"><i><u>BBa_K208001</u></i></font></a> from the Utah 2009 team. The sequence  
 
<p>The sequencing of this part is difficult because of the repetition of phasin's sequence.</p>
 
<p>The sequencing of this part is difficult because of the repetition of phasin's sequence.</p>
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===Source===
 
===Source===

Revision as of 15:06, 25 October 2010

Phasin-Phasin Intein (for silver standard fusion)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1693
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1279
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is a self cleavable fusion protein. It has been designed thanks to the results of the publication Banki et al. The phasin protein bind to the granule membrane. The second phasin makes the binding stronger and then increases the rate of purified protein during the step of granules extraction. The intein is a self cleavable protein which breaks under PH=6 and allows the release od the fused protein at the end of the sequence


The prefix biobrick standard contains a perfect RBS:,
The suffix is a silver standard fusion suffix :The phasin sequence is the same as the biobrick BBa_K208001 from the Utah 2009 team. The sequence

The sequencing of this part is difficult because of the repetition of phasin's sequence.

Source

Ligation of two synthetized parts : Phasin and Phasin-Intein

References