Difference between revisions of "Part:BBa K404117"
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Constructing viral particles encapsidating the transgene expression cassette flanked by the inverted terminal repeats (ITRs, BBa_K404100 and BBa_K404101) can be used in several applications, such as efficient tumor killing or quantification assays. <br /> | Constructing viral particles encapsidating the transgene expression cassette flanked by the inverted terminal repeats (ITRs, BBa_K404100 and BBa_K404101) can be used in several applications, such as efficient tumor killing or quantification assays. <br /> | ||
The plasmid containing the desired gene of interest is cotransfected with the RepCap plasmid (BBa_K404001, BBa_K404002 or BBa_K404003) and the pHelper plasmid. Several assembly steps have to be performed in order to obtain the fully assembled vectorplasmid. <br/> | The plasmid containing the desired gene of interest is cotransfected with the RepCap plasmid (BBa_K404001, BBa_K404002 or BBa_K404003) and the pHelper plasmid. Several assembly steps have to be performed in order to obtain the fully assembled vectorplasmid. <br/> | ||
Revision as of 03:28, 25 October 2010
[AAV2]-left-ITR_pCMV_betaglobin
| [AAV2-left-ITR_pCMV_betaglobin] | |
|---|---|
| |
| BioBrick Nr. | BBa_K404117 |
| RFC standard | RFC 10 |
| Requirement | pSB1C3 |
| Source | synthetic |
| Submitted by | [http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010] |
Constructing viral particles encapsidating the transgene expression cassette flanked by the inverted terminal repeats (ITRs, BBa_K404100 and BBa_K404101) can be used in several applications, such as efficient tumor killing or quantification assays.
The plasmid containing the desired gene of interest is cotransfected with the RepCap plasmid (BBa_K404001, BBa_K404002 or BBa_K404003) and the pHelper plasmid. Several assembly steps have to be performed in order to obtain the fully assembled vectorplasmid.
Facilitating the cloning steps the iGEM team Freiburg_Bioware 2010 provide the 3' nucleotide components in respect to the desired gene of interest which is the left inverted terminal repeat (BBa_K404100) followed by the CMV promoter (BBa_K404102) and a putative enhancer-element (BBa_K404107).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]