Difference between revisions of "Part:BBa K316006"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K316006 short</partinfo>
 
<partinfo>BBa_K316006 short</partinfo>
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Constructed to be combined with promoter and terminator. The GFP <bbpart>BBa_E0040</bbpart> has a 5'(5xHis) tagg and is linked to XylE <bbpart>BBa_J33204</bbpart> monomer subunit. The linker is composed of a Myc tag (DYKDDDDK), TEV protease recognition sequence ENLYFQG followed by GGSGGS. The purpose of the linker and attached GFP is to render the XylE enzyme inactive, by preventing homo-tetramerization into its functional form.
 
Constructed to be combined with promoter and terminator. The GFP <bbpart>BBa_E0040</bbpart> has a 5'(5xHis) tagg and is linked to XylE <bbpart>BBa_J33204</bbpart> monomer subunit. The linker is composed of a Myc tag (DYKDDDDK), TEV protease recognition sequence ENLYFQG followed by GGSGGS. The purpose of the linker and attached GFP is to render the XylE enzyme inactive, by preventing homo-tetramerization into its functional form.
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Parts were assembled by PCR primer extension for exact methods, see our wiki [http://2010.igem.org/Team:Imperial_College_London/Strategy]
 
Parts were assembled by PCR primer extension for exact methods, see our wiki [http://2010.igem.org/Team:Imperial_College_London/Strategy]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:03, 24 October 2010

N-terminus his tagged-GFP-XylE fusion protein


Constructed to be combined with promoter and terminator. The GFP BBa_E0040 has a 5'(5xHis) tagg and is linked to XylE BBa_J33204 monomer subunit. The linker is composed of a Myc tag (DYKDDDDK), TEV protease recognition sequence ENLYFQG followed by GGSGGS. The purpose of the linker and attached GFP is to render the XylE enzyme inactive, by preventing homo-tetramerization into its functional form.


Parts were assembled by PCR primer extension for exact methods, see our wiki [http://2010.igem.org/Team:Imperial_College_London/Strategy]


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1106
    Illegal NgoMIV site found at 1278
    Illegal AgeI site found at 1629
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662