Difference between revisions of "Part:BBa K316006"
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K316006 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K316006 SequenceAndFeatures</partinfo>
  

Revision as of 17:03, 24 October 2010

N-terminus his tagged-GFP-XylE fusion protein

Constructed to be combined with promoter and terminator. The GFP BBa_E0040 has a 5'(5xHis) tagg and is linked to XylE BBa_J33204 monomer subunit. The linker is composed of a Myc tag (DYKDDDDK), TEV protease recognition sequence ENLYFQG followed by GGSGGS. The purpose of the linker and attached GFP is to render the XylE enzyme inactive, by preventing homo-tetramerization into its functional form.

Parts were assembled by PCR primer extension for exact methods, see our wiki [http://2010.igem.org/Team:Imperial_College_London/Strategy]

Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1106
    Illegal NgoMIV site found at 1278
    Illegal AgeI site found at 1629
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662