Difference between revisions of "Part:BBa K389413:Design"

Latest revision as of 14:43, 17 October 2010

vir promoter + sensitivity tuner + luciferase

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2455
Illegal NgoMIV site found at 3799
Illegal NgoMIV site found at 3820
Illegal AgeI site found at 1978
Illegal AgeI site found at 2090
Illegal AgeI site found at 2292
Illegal AgeI site found at 3523
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 3705

a modified sensitivity tuner BBa_I746390 to enhance the luciferase signal which interacts as a reporter for a vir promoter

luciferase from pGL4.10[luc2] (Promega) (BBa_K389004)
terminator, RBS and constitutive promoter from the parts.igem (BBa_B0017, BBa_B0034, BBa_J23110)
sensitivity tuner part consists inter alia of
- delta activator from phage phiR73 (BBa_I746352)
- PF promoter from phage P2 (BBa_I746360)
vir promoter from Agrobacterium tumefaciens C58, TI-plasmid (BBa_K389003)

[http://2007.igem.org/Cambridge/Amplifier_project Cambridge iGEM Team wiki 2007, amplifier project]

@@ Line 10: / Line 10: @@
 ===Source===
-* luciferase from pGL4.10luc2 (Promega) (<partinfo>K389004</partinfo>)
+* luciferase from pGL4.10[luc2] (Promega) (<partinfo>K389004</partinfo>)
 * terminator, RBS and constitutive promoter from the parts.igem (<partinfo>B0017</partinfo>, <partinfo>B0034</partinfo>, <partinfo>J23110</partinfo>)
 * sensitivity tuner part consists inter alia of
@@ Line 18: / Line 18: @@
 ===References===
-[http://parts.mit.edu/igem07/index.php/Cambridge/Amplifier_project Cambridge iGEM Team wiki 2007, amplifier project]
+[http://2007.igem.org/Cambridge/Amplifier_project Cambridge iGEM Team wiki 2007, amplifier project]