Difference between revisions of "Part:BBa K389423:Design"
| Line 10: | Line 10: | ||
===Source=== | ===Source=== | ||
| − | * luciferase from pGL4. | + | * luciferase from pGL4.10[luc2] (Promega) (<partinfo>K389004</partinfo>) |
* terminator, RBS and constitutive promoter from the parts.igem (<partinfo>B0017</partinfo>, <partinfo>B0034</partinfo>, <partinfo>J23110</partinfo>) | * terminator, RBS and constitutive promoter from the parts.igem (<partinfo>B0017</partinfo>, <partinfo>B0034</partinfo>, <partinfo>J23110</partinfo>) | ||
* sensitivity tuner part consists inter alia of | * sensitivity tuner part consists inter alia of | ||
| Line 19: | Line 19: | ||
===References=== | ===References=== | ||
| − | [http:// | + | [http://2007.igem.org/Cambridge/Amplifier_project Cambridge iGEM Team wiki 2007, amplifier project] |
Latest revision as of 14:42, 17 October 2010
VirA/G signaling system + sensitivity tuner + luciferase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 647
Illegal NheI site found at 2581
Illegal NheI site found at 2604 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1632
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 5029
Illegal NgoMIV site found at 6373
Illegal NgoMIV site found at 6394
Illegal AgeI site found at 4552
Illegal AgeI site found at 4664
Illegal AgeI site found at 4866
Illegal AgeI site found at 6097 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1768
Illegal SapI.rc site found at 6279
Design Notes
a modified sensitivity tuner BBa_I746390 to enhance the luciferase signal which interacts as a reporter for a VirA/G signaling system
Source
- luciferase from pGL4.10[luc2] (Promega) (BBa_K389004)
- terminator, RBS and constitutive promoter from the parts.igem (BBa_B0017, BBa_B0034, BBa_J23110)
- sensitivity tuner part consists inter alia of
- delta activator from phage phiR73 (BBa_I746352)
- PF promoter from phage P2 (BBa_I746360)
- virG was synthesized by Mr. Gene (BBa_K389002)
- virA and vir promoter from Agrobacterium tumefaciens C58, TI-plasmid (BBa_K389001, BBa_K389003)
References
[http://2007.igem.org/Cambridge/Amplifier_project Cambridge iGEM Team wiki 2007, amplifier project]