Difference between revisions of "Part:BBa K389411:Design"

Latest revision as of 14:42, 17 October 2010

vir promoter + sensitivity tuner + luciferase

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2428
Illegal NgoMIV site found at 3772
Illegal NgoMIV site found at 3793
Illegal AgeI site found at 1951
Illegal AgeI site found at 2063
Illegal AgeI site found at 2265
Illegal AgeI site found at 3496
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 3678

a modified sensitivity tuner BBa_I746370 to enhance the luciferase signal which interacts as a reporter for a vir promoter

luciferase from pGL4.10[luc2] (Promega) (BBa_K389004)
terminator, RBS and constitutive promoter from the parts.igem (BBa_B0017, BBa_B0034, BBa_J23110)
sensitivity tuner part consists inter alia of
- ogr activator from phage P2 (BBa_I746350)
- PF promoter from phage P2 (BBa_I746360)
virG was synthesized by Mr. Gene (BBa_K389002)
vir promoter from Agrobacterium tumefaciens C58, TI-plasmid (BBa_K389003)

[http://2007.igem.org/Cambridge/Amplifier_project Cambridge iGEM Team wiki 2007, amplifier project]

@@ Line 10: / Line 10: @@
 ===Source===
-* luciferase from pGL4.10luc2 (Promega) (<partinfo>K389004</partinfo>)
+* luciferase from pGL4.10[luc2] (Promega) (<partinfo>K389004</partinfo>)
 * terminator, RBS and constitutive promoter from the parts.igem (<partinfo>B0017</partinfo>, <partinfo>B0034</partinfo>, <partinfo>J23110</partinfo>)
 * sensitivity tuner part consists inter alia of
@@ Line 19: / Line 19: @@
 ===References===
-[http://parts.mit.edu/igem07/index.php/Cambridge/Amplifier_project Cambridge iGEM Team wiki 2007, amplifier project]
+[http://2007.igem.org/Cambridge/Amplifier_project Cambridge iGEM Team wiki 2007, amplifier project]